What can cause hemolysis of red blood cells during blood collection?

When blood is collected, hemolysis refers to the rupture of red blood cells, releasing their contents into the plasma. This can lead to various complications, including inaccurate lab results. One of the common causes of hemolysis during blood collection is excessively shaking or rotating the blood collection device. This vigorous agitation creates shear forces that can damage the fragile red blood cell membrane, leading to hemolysis.

Proper handling techniques, such as gently mixing blood in tubes, are critical to avoid introducing stress that causes cell rupture. Within the context of the choices provided, while other options may relate to blood collection practices, none contribute to hemolysis as significantly as the act of excessive shaking or rotation does. It is essential for phlebotomy technicians to handle blood samples with care to maintain the integrity of the specimen for accurate diagnosis and treatment planning.